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TimJ

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COD Fractionation
Apr 16^th, 2011, 8:00am

Hi everyone, I am currently studying a doctorate, investigating wastewater, algae and co2 sequestration. I want to look at cod fractionation, however the only equipment I have is a COD and BOD kit and a DO probe. Please can anyone recommend a method for fractionation, using this equipment? Also I want to simulate wastewater and COD levels. Can anyone recommend a suitable recipe for the wastewater and how to increase COD (I am currently thinking about using glucose or acetate). Thankyou in advance. Tim

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David S.

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Re: COD Fractionation
Reply #1 - Apr 18^th, 2011, 8:27am

What exactly do you mean by 'COD Fractionation'? Which types of fractions are you looking for? The most reliable way to increase COD is by the use of potassium acid phthalate (KHP). That is the salt that is used to make COD standards. I'm not sure on a synthetic wastewater mix. You might be able to find something in Standard Methods for the Examination of Water and Wastewater.

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TimJ YaBB Newbies Offline I love Water and Wastewater.com Posts: 7 Company or Organization: Birmingham City University	Re: COD Fractionation Reply #2 - Apr 18^th, 2011, 9:50am Hi David, Thank you for your reply. I want to look at creating a COD profile, investigating both biodegradable and non degradable fractions so I can see how my algae are affected by it. As regards the KHP, I did look at it but I don't know if the algae will readily break this down Tim
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Re: COD Fractionation
Reply #3 - Apr 18^th, 2011, 10:17am

If you want biodegradable vs. non COD, you should take a total COD reading of your sample. Then you need to set some aliquots of your sample up for BOD. You will need to decide if you want to go with a traditional 5 day BOD or do an ultimate BOD. Then take a COD reading after the BOD is complete. The final reading should be just the non biodegradable portion. This should help you separate the COD into the two fractions you want. You may need to correct for the nutrients and seed material added during the BOD test. Just do a COD of the dilution water with and without seed added.

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Victor

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Re: COD Fractionation
Reply #4 - Apr 18^th, 2011, 11:32am

Tim:

You may want to try accessing the following paper:

"Rapid Physical-Chemical Method for the Determination of Readily Biodegradable Soluble COD in Municipal Wastewtater" by Daniel Mamais et al, Wat. Res. Vol. 27, No. 1, pp 195-197

For the synthetic wastewater you may want to take a look at a previous posting:

http://www.waterandwastewater.com/cgi-bin/yabb/YaBB.pl?board=general;action=display;num=1152815032

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TimJ YaBB Newbies Offline I love Water and Wastewater.com Posts: 7 Company or Organization: Birmingham City University	Re: COD Fractionation Reply #5 - Apr 18^th, 2011, 12:00pm Thank you David for your help. That's really helped me a lot. I can now concentrate on trying to understand the principles of total alkalinity. Kind regards Tim
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TimJ YaBB Newbies Offline I love Water and Wastewater.com Posts: 7 Company or Organization: Birmingham City University	Re: COD Fractionation Reply #6 - Apr 18^th, 2011, 12:01pm Thank you as well Victor for the help. Tim
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Re: COD Fractionation
Reply #7 - Apr 18^th, 2011, 8:00pm

Tim:

I am perplexed and scratching my head at the same time. Taking on a broad subject matter (photosynthesis, CO2 utilization, wastewater) and having little foundation to start your PhD dissertation? This is a multidisciplinary task with chemistry, biological, and laboratory foundations that may be a bit too late to take in through the process of osmosis or attempting to understand the implications through a Forum. Nevertheless, you have a formidable undertaking.

1. carbonate/bicarbonate/OH alkalinity equilibrium with pH correlation
2. P limits algal growth (you may have all the nutrients/micronutrients present but if P is not found in a biologically active form you will not have algal growth/reproduction)
3. Turbidity/color will interfere with photosynthesis
4. Only certain alga will tolerate wastewater environment

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TimJ YaBB Newbies Offline I love Water and Wastewater.com Posts: 7 Company or Organization: Birmingham City University	Re: COD Fractionation Reply #8 - Apr 19^th, 2011, 4:14am Hi Victor, Thanks for your reply. I think I have confused you somewhat. I do understand the interactions etc with algae, wastewater co2. In relation to alkalinity I was speaking about the standard methods titration for alkalinity. Yes it is a formidable task and there aren't enough hours in the day. Thanks for your help. Tim
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sludge judge Hennessy Ex Member	Re: COD Fractionation Reply #9 - Apr 19^th, 2011, 1:52pm http://dnr.wi.gov/org/water/wm/ww/biophos/3fract.htm not sure if this helps or not, may be worth a look
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TimJ YaBB Newbies Offline I love Water and Wastewater.com Posts: 7 Company or Organization: Birmingham City University	Re: COD Fractionation Reply #10 - Apr 20^th, 2011, 5:00am Hi thanks for the link, Tim
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Re: COD Fractionation
Reply #11 - Apr 23^rd, 2011, 4:44pm

Tim:

Would be curious to know the outcome of your study when done. Always looking to see where a new method will readily replace current understanding. You may want to take it a bit further and use the alga for creating biofuel (which a few Universities are doing in conjunction with exhaust gases from coal fired power plants) or as a feed for grazing animals. Unfortunately, the use of alga requires large quantities of water--reclaimed water may be an option. Sunlight limits where in the US this can take place.........

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TimJ YaBB Newbies Offline I love Water and Wastewater.com Posts: 7 Company or Organization: Birmingham City University	Re: COD Fractionation Reply #12 - Apr 27^th, 2011, 5:03am Hi Victor, Yes I will keep in touch, definately. As regards biofuels, we will be studying lipids and biomass calorific values etc. As regards the solar budget, yes Britain seems to suffer from a distinct lack of sun!! However over the last few weeks I have managed to develop a good tan. Thanks for your help. Tim
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