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May 22nd, 2013, 4:46pm
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Seeding Sludge (Read 663 times)
Henry101
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Seeding Sludge
Jan 06th, 2012, 12:58am
 
I am about to load a batch reactor with RAS sludge supplied from a municipal works to seed it. The sludge will be collected from the evening before and brought over n the morning. I'm guessing that this would mean some of it sitting unaerated for up to 12 hours

- Will this significantly harm the biomass?

- what max time in storage/transport would you recommend?

-on arrival, would you recommend strong feeding and aertion, or slowly slowly?

Ant tips on thi part o the process apperciated. Henry
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dwi handaya
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Re: Seeding Sludge
Reply #1 - Jan 6th, 2012, 2:34am
 
I just finished a start up WWT with success. Clue for seeding sludge

1. Control FM ratio in the safe range (low rate as BOD) 0.08 - 0.15

2. Check microscopic of sludge in sludge storage tank, by general keeping sludge 3 - 7 days still okay. Less will better.

3. Slowly feeding depend on the FM ratio, increasing COD load base on max error 20 % from COD load last 5 days average
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Re: Seeding Sludge
Reply #2 - Jan 6th, 2012, 8:53am
 
Hi Henry,

12 h should be no problem. The lower the sludge loading rate on the donor plant, the longer the sludge can stay unaerated. I personally would not go over three days anaerated time

as Dwi suggested, it is smart to have the donor sludge microscopically checked for filaments. You do not want to import problems..

Upon arrival, i would give some good aeration (DO above 3 mg/L) for at least 0.5 to 1 hour, to wake the bugs up. The feeding level/regime: that is a good question... depends upon the sludge and where it came from (inversely, this may be a criterion to choose the donor plant!). Doing something similar as what it was used to looks like a good starting regime/strategy and go to a situation/regime where the recipient plant will work at after a couple of weeks after the start-up (sludge loading rate, feeding pattern ,...)
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Re: Seeding Sludge
Reply #3 - Jan 6th, 2012, 5:04pm
 
concur with the above, make sure to do a good microscopy exam of the seed prior to intiating feed to ensure you aren't importing an unknown headache from the source.


Dallas
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Have a great day,

Dallas
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Henry101
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Re: Seeding Sludge
Reply #4 - Jan 7th, 2012, 2:07am
 
Many thanks for your replies, a further question has come up; the guy arranging the sludge supply now proposes to switch the source to a brewery WWTW - this my be more suitable as I guess the waste ther is carbon-rich and nutrient poor which would suit this site - but maybe top up with RAS from the municipal TW.

Apart from increasing the risk of filaments, importing fron 2 sources - is there any other downside to this? or might the added diversity help?

thanks, henry
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Re: Seeding Sludge
Reply #5 - Jan 7th, 2012, 3:41pm
 
It shouldn't make a big difference, the imported bacteria won't dictate what grows in the plant, the conditions in your plant will. (You are what you eat!)

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Re: Seeding Sludge
Reply #6 - Jan 11th, 2012, 2:08am
 
Sludge Bacterial Seeding is often used to boost the biological system, or in situations when the whole procedure activities complete failing and incapable to malfunction natural waste properly.
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Re: Seeding Sludge
Reply #7 - Jan 12th, 2012, 3:49am
 
Hi Kyle,

this is about wastewater, not agriculture.
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Re: Seeding Sludge
Reply #8 - Jan 15th, 2012, 6:55am
 
Hello again,

ok so we managed to get hold of just enough sludge to give us a starting mlss of 1500 and want to grow it to around 8000 - problem is the factory ( food packaging, carbohydrate and fats) is well short of full production and we are well under the predicted COD and volumes of influent, with a limited time to get the plant up and running.

I don't have enough food for my poor microbes!

Can anyone suggest an emergency feedstock thet can be easily added to get things moving until the factory produces more waste?

thanks.

hebry
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Re: Seeding Sludge
Reply #9 - Jan 15th, 2012, 2:19pm
 
Can you provide some analytical info of how underloaded you actually are- when is the influent supposed to return to normal? What is normal?

If these answers aren't known I'd almost be inclined to establish a consistent sludge age for now, and when the loading increases back off on wasting until you get to your target MLSS concentration.

Otherwise dog food is a good choice- cheap and good nutrient ratio. Acetate/ sugar/ methanol are other options.

Why a target MLSS of 8,000?

best regards,

Ryan
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Re: Seeding Sludge
Reply #10 - Jan 15th, 2012, 2:20pm
 
or, maintain the biomass you have now at the low loading and when the loading increases seed again to help boost the F/M quicker (stop wasting at that point too and slowly build back up)

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Re: Seeding Sludge
Reply #11 - Jan 20th, 2012, 5:29am
 
Thanks for your [posts, and sorry for the late reply Ryan - been chasing my own tail ( maybe I could do with the dog food?).

Incoming load is around 400 mg/l COD, full predicted around 1100, also this is only over 10 hors a day whilst predicted was on 24 hour operation, so some day only 8-10 kg COD a day against nearly 100 predicted!

ok so we only have arouns 1600 mg/l in 110 m3 at present but tring to biuld. mlss value is needed to run an MBR

client has asked question what if factory closes how do we maintain? so acetate tank maybe the answer.

Expect to re-seed/ boos biomass short;y too ( but that ill men even more biomass to maintain...?
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Re: Seeding Sludge
Reply #12 - Jan 20th, 2012, 6:06am
 
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Henry101
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Re: Seeding Sludge
Reply #13 - Jan 21st, 2012, 5:49am
 
Not sure how the previous post fits in to this topic ?

Have bought some dog food - large adult mix - any idea on figures for kg COD/kg dog food?

biomass is suffering today, mlss has dropped below 1500 and settles less well - we have started recycle and the pump is very fierce, fear it may be smashing the floc! either that, or the addition of waswater effluent is introducing biocides - any further ideas folks?

Henry
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Re: Seeding Sludge
Reply #14 - Jan 22nd, 2012, 10:18am
 
What I'd do is add a known weight of dog food to a known volume of water. (DI water or influent in which you know the COD). Let the dogfood dissolve (buy the soft stuff), mix it up and test the COD. From there you can do the calculations on how much to add for desired lbs of COD to feed the plant.

Just speculation, but I'd imagine the settling is fast with a turbid supernatant (pin floc) which is causing the turbidity. If you are recycling the effluent I wouldn't worry about the pump/ damage to the flocs (in the TSS)- they'll have plenty of time to recover in the aeration tank. I don't see how recycling a non-toxic effluent would introduce toxicity.

What does your wasting schedule/program look like? Could you describe it?

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Re: Seeding Sludge
Reply #15 - Jan 27th, 2012, 1:45pm
 
ballpark answer: 1 lb dogfood: 1 lb COD

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Re: Seeding Sludge
Reply #16 - Mar 31st, 2012, 12:17am
 
The conventional strategy to start up of municipal systems is to use sludge brought in from another plant. However there are many disadvantages with this option such as protection, acclimation, composition, transport & cost.
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