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Jun 19th, 2013, 12:34am
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Initial DO (Read 135 times)
A Beak
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Initial DO
Feb 17th, 2012, 10:41am
 
I'm hoping some can clarify this for me.  I was always taught that when setting up BOD you do not record a DO higher than 9.2.  I have looked through several books about BOD's as well as Standard Methods and I havn't seen anything about a minium initial DO.  Any words of advice on this?  The bottles i Just set up ranged from 9.1 to 9.6 mg/L.  Thanks
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Re: Initial DO
Reply #1 - Feb 17th, 2012, 2:22pm
 
This website may help you to understand the variables affecting DO solubility in water. In my experience, I would recalibrate the DO meter and check the solubility under your conditions.

http://antoine.frostburg.edu/chem/senese/101/solutions/faq/predicting-DO.shtml

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A Beak
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Re: Initial DO
Reply #2 - Feb 17th, 2012, 2:28pm
 
Thanks Grrun.  I don't think that it was a calibration issue.  At least, i HOPE its not.  I just purchased a brand new LDO meter for our BODs and this was my first time using it.  I followed the calibration directions exactly.
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Re: Initial DO
Reply #3 - Feb 17th, 2012, 4:39pm
 
You need to make sure that your samples are at 20 C before getting the initial DO.  SM 21st edition does not give a specific number for the initial DO, but says "Samples containing DO concentration above saturation at 20°C may be encountered in cold waters..." [5210B 4. b. 4)] So you get an upper limit of whatever saturation may be at your temperature and pressure.  I think 9.15 is saturated at sea level pressures.  Depending on your DO meter and preferred significant digits that could be 9.1 or 9.2.  To reduce supersaturation, get the sample to the aforementioned 20 C, half fill a BOD bottle with the sample, put the stopper in, and do your best dance club moves (in other words, shake it up).  Don't worry about undersaturating.  This is a purely mechanical equilibrium and you won't be able to overshoot.

As far as a minimum DO requirement goes, we always stuck with 7.00 mg/L.  The dilution water has a requirement of at least 7.5 mg/L (SM 21st 5210B 5. a.)  We decided that approximately a 5% reduction was acceptable and rounded that to the nearest whole number.  Basically you just want to ensure that you have enough DO to allow for appropriate depletion.  You have the minimum depletion requirement of 2.0 mg/L, which pushes you down to 5.0 if you start with 7.0.
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