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Jun 18th, 2013, 8:34pm
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BOD Calculation (Read 1697 times)
sruthi
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BOD Calculation
Apr 23rd, 2012, 3:08am
 
Hai everyone,

I am getting confused with BOD calculation(Seeded).

Any body can give simple explanation of this calculation  and procedure ?

Thanks for the help
Sruthi
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David S.
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Re: BOD Calculation
Reply #1 - Apr 23rd, 2012, 8:24am
 
The first thing that you need to do is calculate the seed correction factor.  This is determined by setting up several bottles with large volumes of seed added to them.  The easiest way, in my opinion, is to use three bottles containing three, four, and five times the volume of seed that is added to your samples.  For example, if you add 3 mL of PolySeed to your BOD samples, you would add 9, 12, and 15 mL seed to your seed control bottles.  You incubate these bottles just the same as the samples and they have the same acceptance criteria.  Just for the sake of example I'll throw some numbers out there using the conditions I outlined.  

Seed control with 9 mL - initial DO 8.64  final DO 6.06 depletion= 2.58
Seed control with 12 mL - initial DO 8.59 final DO 5.41 depletion= 3.18
Seed control with 15 mL - initial DO 8.62 final DO 3.99 depletion= 4.63

You take each depletion amount and divide by the amount of seed added to the samples, in our example 3 mL.  This gives you (rounded to 2 significant digits) 0.86, 0.80, and 0.93 with an average value of 0.86.  This is your seed correction factor (SCF).
Now for the sample calculation.  Your sample had an initial DO of 8.67 and a final DO of 2.45.  The bottle contained 150 mL of sample.  Your calculation becomes [(Initial DO - Final DO)- SCF]*300/Sample Volume (mL) = [(8.67-2.45)-0.86]*300/150 = 10.72 mg/L.
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sruthi
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Re: BOD Calculation
Reply #2 - Apr 27th, 2012, 11:58pm
 
Thanks Mr David.
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nasrin
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Re: BOD Calculation
Reply #3 - Jun 10th, 2012, 11:08pm
 
thank you Mr David S.
your reply was useful for me too!!
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Re: BOD Calculation
Reply #4 - Jul 17th, 2012, 1:11pm
 
I've been trying to determine the BOD of  a beauty cream. After making some research I decided for the following methods:

1. Dissolve 1 g. of cream in 100 ml of destiled water.
2. Take 50 ml of this dilution and mix with 50 ml of domestic seewage water.
3. Measure the BOD in a respirometer. I take 21 ml of the second solution and measure in a 0-4000 mg/L range, I also add nitrification inhibitor and KOH for CO2 uptake, as the respirometer method requires. I also measure the BOD of the sewage in a different range (0-800 mg/L)

I finally get good results, but the big question is how will I determine the BOD value of the cream?
Since BOD value is independent of the volume I guess it will be easy to calculate by:
BOD of cream = BOD of solution #3 - BOD of domestic seewage

But, should I considerate a seed correction?
And more over, since the cream is dissolve in water I guess I should calculate the dilution factor, in order to multiply the BOD with this factor.


Thank you, Smiley
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Re: BOD Calculation
Reply #5 - Jul 17th, 2012, 1:59pm
 
I am not as familiar with respirometric BOD as I am with the normal 5 day, but I'll give this a shot. I presume you mixed the cream/water solution with domestic sewage to give it a bacteria population to work with. If so, that has become your seed source. The calculation is [oxygen uptake of sample-oxygen uptake of sewage*(0.5)]*(1000/actual volume of sample). In your case I guess you would substitute the actual volume of sample for the actual mass of the sample, 0.005 g in your case. You might try to get a density of the cream to convert it to volume, or presume a density near 1.0.
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Re: BOD Calculation
Reply #6 - Aug 1st, 2012, 3:01am
 
Hi All,
Am  a new member to this forum.
Am currently working in a water Lab and came across VELP scientifica BOD incubator,.
I find the manual abit shallow and could not give more details on how to choose sample volume, scale and final reading after 55  days.
Is there any one who used this equipment?
How can i get a good result?

Thank you
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